Proteus mirabilis tsi results
TRIPLE SUGAR IRON AGAR: INTERPRETATION OF RESULTS page 55 David B. Fankhauser, Ph. D. rvsd 26 July 1994, rvsd 23 July '95, 23 July '96, 15 July 01, 28 June 02, 7 Aug 02, 23July09, 22July10 for a given set of TSI traits, there are many more possible species than only those listed. Proteus mirabilis R() YYG blackgreen butt, slant canTriple Sugar Iron Agar (TSI): Principle, Procedure and Interpretation July 16, 2013 tankeshwar Biochemical tests in Microbiology, laboratory diagnosis of Bacterial Disease 77 Triple Sugar Iron Agar Test Results Image source: Clark College proteus mirabilis tsi results
Tsi test. Utilization of peptones results alkalization of the medium due to the production of NH3. The production of hydrogen sulfide is indicated by the presence of black ppt. formed by the reaction of H2S with ferric ions. Slant is aerobic while butt is anaerobic. Gas production is indicated by the splitting of the agar of lifting
Nutrient agar is always a safe choice. MacConkey is usually fairly decent, especially if you want to test the lactose fermentation thing. Finally, blood agar is always really cool for p. mirabilis, because the organism has a tendency to swarm across the agar (because of its high motility), so thats cool to see. Appearace of Enteric Organisms on Triple Sugar Iron Medium. These organisms use the protein at the aerobic surface of the slant as a carbon source, the pH increases and the top of the slant becomes red. The black is due to hydrogen sulfide production. Substantial hydrogen sulfide production by Proteus mirabilis makes it difficult to determine colorproteus mirabilis tsi results TSI Agar is a modification of the classical Kligers agar. 1 sucrose has been added to this medium to differentiate and ProteusHafnia (sucrose positive) from Salmonella and Shigella (sucrose negative).
Lab Final Gram Negative Rods Tests Learn with flashcards, games, and more for free. Diff between Providencia stuartii and Proteus vulgaris is TSI (no sulfur), Citrate POS for Providencia and no test for Proteus, PADNEG, and Flagellar Stain for Proteus Proteus mirabilis. rod Indole Negativediff between Proteus mirabilis and Proteus proteus mirabilis tsi results Hajna developed the formulation for TSI Agar by adding sucrose to the double sugar (glucose and lactose) formulation of Kligler Iron Agar. 1, 2 Triple Sugar Iron Agar contains three carbohydrates (glucose, lactose and sucrose). When these carbohydrates are fermented, the resulting production of acid is detected by the phenol red indicator. Proteus mirabilis appears as Gramnegative rods after Gram staining under brightfield microscopy with 1000 times magnification. Proteus mirabilis is a Gramnegative, facultatively anaerobic, rodshaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90 of all Proteus infections in humans. Spositive bacterias reduce the sulfite in the culture medium to sulfide, which reacts with iron. Due to this reaction a black precipitate (FeS) is formed. Triple Sugar Iron Agar should be used in parallel with Urea Broth ( ) to distinguish between Salmonella and Proteus species.Rating: 4.42 / Views: 478